Antibody

Antibody

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Month: April 2018

Overcome Infertility – Treating Anti-Sperm Antibody in Conventional Perspective

Posted on April 27, 2018 in Uncategorized

As we mentioned in previous article, conventional medicine plays an important role and most of the time is the first treatment for a couple who for what ever reason cam not conceive after 1 year of unprotected sexual intercourse or can not carry the pregnancy to full term. With the advance of medical technology, conventional medicine has proven record in treating infertility but with some side effects. In this article, we will discuss Laparotomy microsurgery: definition, effects, side effects and risk of conventional medication in treating hormone imbalance.

1. Definition
Antisperm antibody can be produced by either partner. It is defined as a certain class of protein attaches themselves to the sperm, causing other cells in the immune system to attack them, leading to low quality sperm in men and effecting the ability of sperm to fertilize the egg in women.

2. Causes
Since the protein can attach to the sperm, it inhibits the sperm function in egg fertilization
a) Blood contamination
A Man may produce antisperm antibody, if he injures to his testicle or due to surgery such as undescent testicle surgery, testicle biopsy, testicle torsion, etc.
b) Break down of the test barrier
Normally sperm are kept separate from blood and immune system. If there are damage to the barrier for what ever reason, the immune system may recognize the sperm as the foreign intrusion and destroy them.
c) When it is formed
For what ever reasons, antisperm antibody can be formed due to infection, inflammation or medicine, etc. If it exist, it may interferes with quality of sperm in men and make cervical mucus hostile to sperm invasion, leading to infertility.

3. Treatments
a) Corticosteroids
Corticosteroids is a drug which normally used to treat any swelling condition such as skin problems, severe allergies, asthma, and arthritis. It works effectively in reducing the production of antisperm antibody, but it causes serious side effect including make infections harder to treat, nervousness, restlessness, sleep problems, and indigestion.
b) Sperm treatments that use for ertificila insemination such as rapid washing and freeze-thawing.

Suspecting Candidiasis – Elevated Antibodies Causing EBV

Posted on April 21, 2018 in Uncategorized

Someone I know has heard of candidiasis in the past and wonder if she is a suspect. Well, she is 99.9% sure of it. Although she is a thin child, she was fed ice cream and sweets every night. She took antibiotics for acne from ages 10-17. At age 17, she started birth control pills. She is now 28, and she thinks she is falling apart! She needs to lose some weight, but more importantly, she just nuts certain weeks out of her cycle. This is getting worse and worse. Plus, she is a major sugar addict. It is her drug of choice. Also, she has had mononucleosis three times in her life which leads her to believe that there is something more going on such as CFS or EBV.

Do you have the same situation as hers? In the matter of EBV, you probably have elevated antibodies, and while viruses cannot be cured, they can be suppressed. The next time you see your doctor, I would ask him to do an antibody test for EBV. Actually, if he did a molecular test, it would show if you had an active infection, but most doctors would tell you such a test is ridiculous. To lower your antibodies, you can take one of many immune enhancing products that will build up your NKCs (natural killer cells) which in turn will battle to reduce the EBV antibodies.

By the way, I take Immune Support Factors by Chisholm labs, but there are tons of others available. You pretty much have to go through a trial and error process, because what works for one person may not work for another.

Antibody Engineering For Specific Application

Posted on April 15, 2018 in Uncategorized

Nowadays pharmaceutical and medicinal biotechnology is an improved section of biotechnology. Production of different developed drugs, vaccines, artificial insulin, etc. are achievements of this section. Antibody engineering is one of the most developed parts of medicinal biotechnology. If an antibody is modified due to a specific application through recombinant DNA technology then it is called antibody engineering. It is also called hybrid antibody or recombinant antibody.

Some steps which are essential for doing antibody engineering are-

1. Development of a design that will prepare the antibody to be used for a specific application that means by which it will bind with a specific antigen.

2. Accumulation of all the DNA sequences that would generate this antibody into a suitable expression vector

3. Introduction of a vector in a myeloma cell line so that the modified antibody gene can express itself

4. Mass culture of the transfected clones containing modified antibody genes of myeloma cell line

5. Harvesting the produced recombinant antibodies. But it should be noticed that the myeloma cell line would not produce any antibody of its own.

Monoclonal antibody that is being used widely as therapeutic agent has been produced by using antibody engineering. For producing monoclonal antibody a myeloma cell line is fused with spleen cell line of mouse which has already immunized with the desired antigen. In this HAT medium is used as culture medium because it contain hypoxanthine, thymine and aminopterin. Unfused hybridoma cell cannot grow in HAT medium due to lack of HGPRT and unfused spleen cell cannot grow infinitely because they have limited life span. After producing monoclonal antibody it is purified by western blotting system. Now a day’s monoclonal antibodies are widely being used in cancer treatment. It is also used for treating autoimmune diseases like infliximab, adalimumab.

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